To observe the effect:: of venom on the early skin injury by Korean Agkistrodon b. brevicudus, Agkistrodon caliginosus and Agkistrodon saxatilis, histopathological studies were done in mice after venom injection by 0.275mg., 0. 175mg. and 0.225mg. subcutaneusly. Mice were sacrificed on 10min; 20min, 30min. G(11min, 2hrs, and 4hrs, after envencmation.
Results obtained were as follows:
1. The effects of Agkistrodon b. brevicaudus venom;
Initial predominant features were moderate to marked dilatation and congestion of venules and
capillaries in dermis and subcutis then followed by edema, hemorrhage and rupture of vessels
pregressively. Fibrin. deposition was not noted. Inflammatory exudates were marked after 2 hours.
2. The effects of Agkistrodon caliginosus venom;
Histological features of vascular changes and inflammatory exudates were similar to those of Agkistrodon b. brez;icaudus by mild degree. Fibin deposition in vessels were noted in two mice.
3. The effects of Agkistrodon saxatilis venom;
Histological features of vascular structure were similar to those of Agkisirodon b: on ica c´us.
inflammatory exudates were marked after 4 hours. Fibrin deposition were seen in 3 mice.
Therefore, the early e;-fects of Korean Agkistrodon venoms on the skin injury were similar and their histopathological changes were characterized by marked telangiectasia and congestion followed by marked hemorrhage, edema and rupture of blood vessels, resulted in localized circulatory disturbances. So direct toxic and later localized circulatory disturbances might play a role in early and progressive skin injury.
|